| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yue Lou, Yaguo L. [Effects of Chlorpyrifos on Dopaminergic Neuronal Viability with Activation of Microglia]. Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi. vol 33. issue 3. 2018-05-24. PMID:29709151. |
excessive microglial activation and subsequent neuroinflammation lead to neuronal cell death,which are involved in the pathogenesis and progression of several neurodegenerative diseases such as parkinson’s disease.the objective of this study was to determine the involvement of chlorpyrifos(cpf)in the activation of microglia and production of inflammatory factors in response to cpf stimulation and the influence on the viability of dopaminergic(da)neurons.we detected the change of bv-2cells morphology and expression of inducible nitric oxide(inos),cyclooxygenase-2(cox-2)mrna and protein level upon cpf stimulation(0,1,3,6,12,24h)in bv-2(mouse brain microglia)cells by reverse transcription polymerase chain reaction(rt-pcr)or western blot.we randomly assigned bv-2cells into cpf,menstruum dimethysulfoxide(dmso)and normal saline(ns)groups.we stimulated the bv-2cells in the cpf group with cpf,and we stimulated the two control groups with dmso or ns for 12 hours,respectively.we then collected the used culture media from the culture dishes and centrifuged it to remove the detached cells.then,we used the supernatants as microglial conditioned media.we treated sh-sy5 yneurons with various groups of microglial conditioned media for 24 hours.we observed the effect of conditioned media collected from bv-2cell on the viability of dopaminergic cell lines sh-sy5 yusing mtt assay.we found that inflammatory factors inos,cox-2mrna and protein levels were up-regulated upon cpf stimulation.conditioned media from bv-2upon cpf stimulation is toxic to sh-sy5 y.it might be concluded that the exposure to cpf may induce dopaminergic neuronal damage by the activation of inflammatory response,and a mechanism may be involved in parkinson’s disease pathogenesis. |
2018-05-24 |
2023-08-13 |
mouse |
| Katherine O'Farrell, Eimear Fagan, Thomas J Connor, Andrew Harki. Inhibition of the kynurenine pathway protects against reactive microglial-associated reductions in the complexity of primary cortical neurons. European journal of pharmacology. vol 810. 2018-04-05. PMID:28688912. |
bv-2 microglia were treated with ifnγ (5ng/ml) for 24h and conditioned media (cm) was placed on primary cortical neurons 3 days in vitro (div) for 48h. |
2018-04-05 |
2023-08-13 |
Not clear |
| Shefang Ye, Tong Zhou, Dongmei Pan, Yiquan Lai, Peiyan Yang, Mingliang Chen, Yange Wang, Zhenqing Hou, Lei Ren, Yuanqin Jian. Fullerene C₆₀ Derivatives Attenuated Microglia-Mediated Prion Peptide Neurotoxicity. Journal of biomedical nanotechnology. vol 12. issue 9. 2018-03-19. PMID:29345893. |
in addition, conditioned culture media taken from prp(106-126)-stimulated microglia cause apoptotic neuronal cell death, which was suppressed by pretreatment with c60-oh. |
2018-03-19 |
2023-08-13 |
Not clear |
| Jia-Hong Chen, Chon-Haw Tsai, Hsiao-Yun Lin, Chien-Fang Huang, Yuk-Man Leung, Sheng-Wei Lai, Cheng-Fang Tsai, Pei-Chun Chang, Dah-Yuu Lu, Chingju Li. Interlukin-18 Is a Pivot Regulatory Factor on Matrix Metalloproteinase-13 Expression and Brain Astrocytic Migration. Molecular neurobiology. vol 53. issue 9. 2018-01-29. PMID:26558633. |
the conditioned medium (cm) collected from activated microglia increased interleukin (il)-18 production and enhanced mmp-13 expression in astrocytes. |
2018-01-29 |
2023-08-13 |
Not clear |
| Valerie Sackmann, Anna Ansell, Christopher Sackmann, Harald Lund, Robert A Harris, Martin Hallbeck, Camilla Nilsbert. Anti-inflammatory (M2) macrophage media reduce transmission of oligomeric amyloid beta in differentiated SH-SY5Y cells. Neurobiology of aging. vol 60. 2018-01-03. PMID:28969867. |
we investigated the effects of conditioned macrophage media, as an analogue to microglia, on the transfer of oligomeric amyloid beta (oaβ) between differentiated sh-sy5y cells. |
2018-01-03 |
2023-08-13 |
Not clear |
| Giuseppina D'Alessandro, Alfonso Grimaldi, Giuseppina Chece, Alessandra Porzia, Vincenzo Esposito, Antonio Santoro, Maurizio Salvati, Fabrizio Mainiero, Davide Ragozzino, Silvia Di Angelantonio, Heike Wulff, Myriam Catalano, Cristina Limatol. KCa3.1 channel inhibition sensitizes malignant gliomas to temozolomide treatment. Oncotarget. vol 7. issue 21. 2017-12-29. PMID:27096953. |
in this paper we demonstrate that: i) blockade of the calcium-activated potassium channel kca3.1 with tram-34 has co-adjuvant effects with tmz, reducing gl261 glioma cell migration, invasion and colony forming activity, increasing apoptosis, and forcing cells to pass the g2/m cell cycle phase, likely through cdc2 de-phosphorylation; ii) kca3.1 silencing potentiates the inhibitory effect of tmz on glioma cell viability; iii) the combination of tmz/tram-34 attenuates the toxic effects of glioma conditioned medium on neuronal cultures, through a microglia dependent mechanism since the effect is abolished by clodronate-induced microglia killing; iv) tmz/tram-34 co-treatment increases the number of apoptotic tumor cells, and the mean survival time in a syngeneic mouse glioma model (c57bl6 mice implanted with gl261 cells); v) tmz/tram-34 co-treatment reduces cell viability of gbm cells and cancer stem cells (csc) freshly isolated from patients.taken together, these data suggest a new therapeutic approach for malignant glioma, targeting both glioma cell proliferating and migration, and demonstrate that tmz/tram-34 co-treatment affects both glioma cells and infiltrating microglia, resulting in an overall reduction of tumor cell progression. |
2017-12-29 |
2023-08-13 |
mouse |
| Chun-Hsien Chu, Shijun Wang, Chia-Ling Li, Shih-Heng Chen, Chih-Fen Hu, Yi-Lun Chung, Shiou-Lan Chen, Qingshan Wang, Ru-Band Lu, Hui-Ming Gao, Jau-Shyong Hon. Neurons and astroglia govern microglial endotoxin tolerance through macrophage colony-stimulating factor receptor-mediated ERK1/2 signals. Brain, behavior, and immunity. vol 55. 2017-12-29. PMID:27132056. |
neutralization of m-csf deprived the neuron-glia conditioned medium of its ability to enable microglia to form et when microglia encountered two lipopolysaccharide (lps) treatments. |
2017-12-29 |
2023-08-13 |
Not clear |
| M Gómez-Cañas, P Morales, L García-Toscano, C Navarrete, E Muñoz, N Jagerovic, J Fernández-Ruiz, M García-Arencibia, M R Pazo. Biological characterization of PM226, a chromenoisoxazole, as a selective CB2 receptor agonist with neuroprotective profile. Pharmacological research. vol 110. 2017-12-21. PMID:27013280. |
conditioned media were collected from lps-stimulated cultures of bv2 microglial cell line in the absence or presence of different doses of pm226, and then media were added to cultured m213-2o neuronal cells to record their influence on cell viability evaluated using mtt assays. |
2017-12-21 |
2023-08-13 |
rat |
| Ming Fang, Yun Yuan, Jia Lu, Hong E Li, Min Zhao, Eng-Ang Ling, Chun-Yun W. Scutellarin promotes microglia-mediated astrogliosis coupled with improved behavioral function in cerebral ischemia. Neurochemistry international. vol 97. 2017-12-12. PMID:27105682. |
in vitro, the above-mentioned proteins were also investigated in tnc 1 and primary astrocytes, treated respectively with conditioned medium from bv-2 microglia with or without pretreatment of scutellarin and lipopolysaccharide. |
2017-12-12 |
2023-08-13 |
rat |
| Ming Fang, Yun Yuan, Jia Lu, Hong E Li, Min Zhao, Eng-Ang Ling, Chun-Yun W. Scutellarin promotes microglia-mediated astrogliosis coupled with improved behavioral function in cerebral ischemia. Neurochemistry international. vol 97. 2017-12-12. PMID:27105682. |
when compared to the controls, tnc1/primary astrocytes responded vigorously to conditioned medium derived from bv-2 microglia treated with scutellarin + lipopolysaccharide as shown by enhanced expression of all the above markers by western and immunofluorescence analysis. |
2017-12-12 |
2023-08-13 |
rat |
| Haitao Shen, Chenglin Liu, Dongping Zhang, Xiyang Yao, Kai Zhang, Haiying Li, Gang Che. Role for RIP1 in mediating necroptosis in experimental intracerebral hemorrhage model both in vivo and in vitro. Cell death & disease. vol 8. issue 3. 2017-11-13. PMID:28252651. |
furthermore, necroptosis of cultured neurons could be induced by conditioned medium from microglia stimulated with oxygen hemoglobin, and this effect could be inhibited by tnf-α inhibitor, indicating that tnf-α secreted from activated microglia is an important factor in inducing necroptosis of neurons. |
2017-11-13 |
2023-08-13 |
rat |
| Xiaojing Lin, Tingbao Zhao, Melissa Walker, Aishi Ding, Shide Lin, Yongcheng Cao, Jinfeng Zheng, Xiaohong Liu, Ming Geng, Xiao-Ming Xu, Shaojun Li. Transplantation of Pro-Oligodendroblasts, Preconditioned by LPS-Stimulated Microglia, Promotes Recovery After Acute Contusive Spinal Cord Injury. Cell transplantation. vol 25. issue 12. 2017-10-30. PMID:27513556. |
we found that prools responded to lipopolysaccharide (lps)-stimulated microglia conditioned medium (l+m) by preserving toll-like receptor 4 (tlr4) expression, improving cell viability, and enhancing the expression of a myelinating ol marker myelin basic protein (mbp), compared to other ol lineage cells exposed to either lps-stimulated (l+m) or nonstimulated microglia conditioned medium (lm). |
2017-10-30 |
2023-08-13 |
Not clear |
| Yoon-Hee Kwon, Jiye Kim, Chu-Sook Kim, Thai Hien Tu, Min-Seon Kim, Kyoungho Suk, Dong Hee Kim, Byung Ju Lee, Hye-Seon Choi, Taesun Park, Myung-Sook Choi, Tsuyoshi Goto, Teruo Kawada, Tae Youl Ha, Rina Y. Hypothalamic lipid-laden astrocytes induce microglia migration and activation. FEBS letters. vol 591. issue 12. 2017-09-25. PMID:28542876. |
medium conditioned by the lipid-laden astrocytes stimulate microglial chemotactic activity and upregulate transcripts of the microglia activation marker iba-1 and inflammatory cytokines. |
2017-09-25 |
2023-08-13 |
Not clear |
| Giovanni Marfia, Stefania Elena Navone, Loubna Abdel Hadi, Moira Paroni, Valeria Berno, Matteo Beretta, Roberta Gualtierotti, Francesca Ingegnoli, Vincenzo Levi, Monica Miozzo, Jens Geginat, Lorenzo Fassina, Paolo Rampini, Carlo Tremolada, Laura Riboni, Rolando Campanell. The Adipose Mesenchymal Stem Cell Secretome Inhibits Inflammatory Responses of Microglia: Evidence for an Involvement of Sphingosine-1-Phosphate Signalling. Stem cells and development. vol 25. issue 14. 2017-09-11. PMID:27217090. |
our results demonstrate that the conditioned medium (cm) of adscs isolated by two different processing methods (lipoaspirate and lipogems) significantly inhibited the lipopolysaccharide (lps)-induced effects on microglia activation, including microglial expression of cd68, cytokine secretion, proliferation, and migration. |
2017-09-11 |
2023-08-13 |
human |
| Kathrin Heiss, Marco Raffaele, Luca Vanella, Paolo Murabito, Orazio Prezzavento, Agostino Marrazzo, Giuseppina Aricò, Carlo Castruccio Castracani, Ignazio Barbagallo, Agata Zappalà, Roberto Avola, Giovanni Li Volt. (+)-Pentazocine attenuates SH-SY5Y cell death, oxidative stress and microglial migration induced by conditioned medium from activated microglia. Neuroscience letters. vol 642. 2017-09-08. PMID:28163081. |
(+)-pentazocine attenuates sh-sy5y cell death, oxidative stress and microglial migration induced by conditioned medium from activated microglia. |
2017-09-08 |
2023-08-13 |
Not clear |
| Pamela J Urrutia, Etienne C Hirsch, Christian González-Billault, Marco T Núñe. Hepcidin attenuates amyloid beta-induced inflammatory and pro-oxidant responses in astrocytes and microglia. Journal of neurochemistry. vol 142. issue 1. 2017-08-14. PMID:28266714. |
hepcidin also reduced neurotoxicity and oxidative damage triggered by conditioned media obtained from astrocytes and microglia treated with aβ. |
2017-08-14 |
2023-08-13 |
mouse |
| Christopher Nebel, Alexander Aslanidis, Khalid Rashid, Thomas Langman. Activated microglia trigger inflammasome activation and lysosomal destabilization in human RPE cells. Biochemical and biophysical research communications. vol 484. issue 3. 2017-06-06. PMID:28159556. |
we found disorganization of arpe-19 cytoskeletal structure after incubation with conditioned media of lps-stimulated microglia and llome and accumulation of lipid deposits in these cells using nile red staining. |
2017-06-06 |
2023-08-13 |
human |
| Yi Feng, Huaqing Xue, Jie Zhu, Likun Yang, Feng Zhang, Rong Qian, Wei Lin, Yuhai Wan. ESE1 is Associated with Neuronal Apoptosis in Lipopolysaccharide Induced Neuroinflammation. Neurochemical research. vol 41. issue 10. 2017-04-04. PMID:27350582. |
in vitro, ese1 depletion in cortical primary neurons inhibited active caspase-3 and bax expression as well as lactate dehydrogenase (ldh) release with up-regulation of bcl-2, while ese1 overexpression can exert opposite effects, indicating that ese1 promoted neuronal apoptosis induced by lps or lps exposed microglia conditioned media (cm). |
2017-04-04 |
2023-08-13 |
Not clear |
| Jeremy T Miyauchi, Danling Chen, Matthew Choi, Jillian C Nissen, Kenneth R Shroyer, Snezana Djordevic, Ian C Zachary, David Selwood, Stella E Tsirk. Ablation of Neuropilin 1 from glioma-associated microglia and macrophages slows tumor progression. Oncotarget. vol 7. issue 9. 2016-12-30. PMID:26755653. |
nrp1-deficient or eg00229-treated wild-type microglia exhibited a shift towards anti-tumorigenicity as evident by altered inflammatory marker profiles in vivo and decreased smad2/3 activation when conditioned in the presence of glioma-derived factors. |
2016-12-30 |
2023-08-13 |
mouse |
| Siqi Zhao, Jingyu Yang, Lixin Wang, Shengyi Peng, Jie Yin, Lina Jia, Xiaowei Yang, Zengqiang Yuan, Chunfu W. NF-κB Upregulates Type 5 Phosphodiesterase in N9 Microglial Cells: Inhibition by Sildenafil and Yonkenafil. Molecular neurobiology. vol 53. issue 4. 2016-12-26. PMID:26108184. |
importantly, sildenafil and yonkenafil significantly alleviated the death of sh-sy5y neuroblastoma cells and primary cortical neurons induced by the conditioned medium from activated microglia. |
2016-12-26 |
2023-08-13 |
Not clear |