| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Berta Puig, Hermann Altmeppen, Markus Glatze. The GPI-anchoring of PrP: implications in sorting and pathogenesis. Prion. vol 8. issue 1. 2015-02-21. PMID:24509692. |
there is growing evidence that toxicity and neuronal damage on the one hand and propagation/infectivity on the other hand are two distinct processes of the disease and that the gpi-anchor attachment of prp(c) and prp(sc) plays an important role in protein localization and in neurotoxicity. |
2015-02-21 |
2023-08-12 |
Not clear |
| Jesús R Requena, Holger Will. The structure of the infectious prion protein: experimental data and molecular models. Prion. vol 8. issue 1. 2015-02-21. PMID:24583975. |
the structures of the infectious prion protein, prp(sc), and that of its proteolytically truncated variant, prp 27-30, have evaded experimental determination due to their insolubility and propensity to aggregate. |
2015-02-21 |
2023-08-12 |
Not clear |
| Jesús R Requena, Holger Will. The structure of the infectious prion protein: experimental data and molecular models. Prion. vol 8. issue 1. 2015-02-21. PMID:24583975. |
here, we present an overview of experimental results that were published in recent years, and which provided new insights into the molecular architecture of prp(sc) and prp 27-30. |
2015-02-21 |
2023-08-12 |
Not clear |
| Jesús R Requena, Holger Will. The structure of the infectious prion protein: experimental data and molecular models. Prion. vol 8. issue 1. 2015-02-21. PMID:24583975. |
moreover, this conclusion constitutes an open invitation for renewed efforts to model the structure of prp(sc). |
2015-02-21 |
2023-08-12 |
Not clear |
| Ilaria Vanni, Michele Angelo Di Bari, Laura Pirisinu, Claudia D'Agostino, Umberto Agrimi, Romolo Nonn. In vitro replication highlights the mutability of prions. Prion. vol 8. issue 1. 2015-02-21. PMID:24618479. |
prions exist as strains, which are thought to reflect prp(sc) conformational variants. |
2015-02-21 |
2023-08-12 |
Not clear |
| Ilaria Vanni, Michele Angelo Di Bari, Laura Pirisinu, Claudia D'Agostino, Umberto Agrimi, Romolo Nonn. In vitro replication highlights the mutability of prions. Prion. vol 8. issue 1. 2015-02-21. PMID:24618479. |
we investigated in vitro prion mutability of 2 strains, by following prp(sc) variations of populations serially propagated in pmca under constant environmental pressure. |
2015-02-21 |
2023-08-12 |
Not clear |
| Ilaria Vanni, Michele Angelo Di Bari, Laura Pirisinu, Claudia D'Agostino, Umberto Agrimi, Romolo Nonn. In vitro replication highlights the mutability of prions. Prion. vol 8. issue 1. 2015-02-21. PMID:24618479. |
in both strains, prp(sc) conformational variants were identified only after large population passages, while repeated bottleneck events caused a rapid decline in amplification rates. |
2015-02-21 |
2023-08-12 |
Not clear |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
ultrasensitive detection of prp(sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
prion diseases are characterized by the prominent accumulation of the misfolded form of a normal cellular protein (prp(sc)) in the central nervous system. |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
the pathological features and biochemical properties of prp(sc) in macaque monkeys infected with the bovine spongiform encephalopathy (bse) prion have been found to be similar to those of human subjects with variant creutzfeldt-jakob disease (vcjd). |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
in the current study, we developed a highly efficient method for in vitro amplification of cynomolgus macaque bse prp(sc). |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
this method involves amplifying prp(sc) by protein misfolding cyclic amplification (pmca) using mouse brain homogenate as a prp(c) substrate in the presence of sulfated dextran compounds. |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
this method is capable of amplifying very small amounts of prp(sc) contained in the cerebrospinal fluid (csf) and white blood cells (wbcs), as well as in the peripheral tissues of macaques that have been intracerebrally inoculated with the bse prion. |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
after clinical signs of the disease appeared in three macaques, we detected prp(sc) in the csf by serial pmca, and the csf levels of prp(sc) tended to increase with disease progression. |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
in addition, prp(sc) was detectable in wbcs at the clinical phases of the disease in two of the three macaques. |
2015-02-13 |
2023-08-13 |
mouse |
| Yuichi Murayama, Kentaro Masujin, Morikazu Imamura, Fumiko Ono, Hiroaki Shibata, Minoru Tobiume, Tomoaki Yamamura, Noriko Shimozaki, Keiji Terao, Yoshio Yamakawa, Tetsutaro Sat. Ultrasensitive detection of PrP(Sc) in the cerebrospinal fluid and blood of macaques infected with bovine spongiform encephalopathy prion. The Journal of general virology. vol 95. issue Pt 11. 2015-02-13. PMID:25024281. |
thus, our highly sensitive, novel method may be useful for furthering the understanding of the tissue distribution of prp(sc) in non-human primate models of cjd. |
2015-02-13 |
2023-08-13 |
mouse |
| Justin J Greenlee, Jodi D Smith, M Heather West Greenlee, Eric M Nicholso. Clinical and pathologic features of H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism. PloS one. vol 7. issue 6. 2015-02-12. PMID:22715405. |
while this isolate is categorized as bse-h due to a higher molecular mass of the unglycosylated prp(sc) isoform, a strong labeling of all 3 prp(sc) bands with monoclonal antibodies 6h4 and p4, and a second unglycosylated band at approximately 14 kda when developed with antibodies that bind in the c-terminal region, it is unique from other described cases of bse-h because of an additional band 23 kda demonstrated on western blots of the cerebellum. |
2015-02-12 |
2023-08-12 |
cattle |
| Nam K Chu, Waheed Shabbir, Erin Bove-Fenderson, Can Araman, Rosa Lemmens-Gruber, David A Harris, Christian F W Becke. A C-terminal membrane anchor affects the interactions of prion proteins with lipid membranes. The Journal of biological chemistry. vol 289. issue 43. 2015-02-12. PMID:25217642. |
membrane attachment via a c-terminal glycosylphosphatidylinositol anchor is critical for conversion of prp(c) into pathogenic prp(sc). |
2015-02-12 |
2023-08-13 |
mouse |
| Lars Luers, Oliver Bannach, Jan Stöhr, Michael Marius Wördehoff, Martin Wolff, Luitgard Nagel-Steger, Detlev Riesner, Dieter Willbold, Eva Birkman. Seeded fibrillation as molecular basis of the species barrier in human prion diseases. PloS one. vol 8. issue 8. 2015-01-22. PMID:23977331. |
the hallmark of prion diseases is the conversion of the host-encoded prion protein (prp(c)) to its pathological isoform prp(sc), which is accompanied by prp fibrillation. |
2015-01-22 |
2023-08-12 |
human |
| Lars Luers, Oliver Bannach, Jan Stöhr, Michael Marius Wördehoff, Martin Wolff, Luitgard Nagel-Steger, Detlev Riesner, Dieter Willbold, Eva Birkman. Seeded fibrillation as molecular basis of the species barrier in human prion diseases. PloS one. vol 8. issue 8. 2015-01-22. PMID:23977331. |
to analyse this process at a molecular level, we previously established an in vitro fibrillation assay, in which recombinant prp (recprp) as substrate can be specifically seeded by prp(sc) as seed. |
2015-01-22 |
2023-08-12 |
human |