| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Zhifu Shan, Takeshi Yamasaki, Akio Suzuki, Rie Hasebe, Motohiro Horiuch. Establishment of a simple cell-based ELISA for the direct detection of abnormal isoform of prion protein from prion-infected cells without cell lysis and proteinase K treatment. Prion. vol 10. issue 4. 2017-11-07. PMID:27565564. |
the analytical dynamic range for prp(sc) detection was approximately 1 log. |
2017-11-07 |
2023-08-13 |
mouse |
| Zhifu Shan, Takeshi Yamasaki, Akio Suzuki, Rie Hasebe, Motohiro Horiuch. Establishment of a simple cell-based ELISA for the direct detection of abnormal isoform of prion protein from prion-infected cells without cell lysis and proteinase K treatment. Prion. vol 10. issue 4. 2017-11-07. PMID:27565564. |
the addition of a cytotoxicity assay immediately before prp(sc) detection did not affect the following prp(sc) detection. |
2017-11-07 |
2023-08-13 |
mouse |
| Zhifu Shan, Takeshi Yamasaki, Akio Suzuki, Rie Hasebe, Motohiro Horiuch. Establishment of a simple cell-based ELISA for the direct detection of abnormal isoform of prion protein from prion-infected cells without cell lysis and proteinase K treatment. Prion. vol 10. issue 4. 2017-11-07. PMID:27565564. |
thus, all the procedures including cell culture, cytotoxicity assay, and prp(sc) detection were completed in the same plate. |
2017-11-07 |
2023-08-13 |
mouse |
| Grit Priemer, Anne Balkema-Buschmann, Bob Hills, Martin H Groschu. Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE. PloS one. vol 8. issue 6. 2017-10-17. PMID:23805320. |
biochemical characteristics and prp(sc) distribution pattern in the brains of cattle experimentally challenged with h-type and l-type atypical bse. |
2017-10-17 |
2023-08-12 |
cattle |
| Grit Priemer, Anne Balkema-Buschmann, Bob Hills, Martin H Groschu. Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE. PloS one. vol 8. issue 6. 2017-10-17. PMID:23805320. |
while the main diagnostic feature of these forms is the altered biochemical profile of the accumulated prp(sc), it was also observed in the initial analysis that l-type bse displays a distribution pattern of the pathological prion protein (prp(sc)), which clearly differs from that observed in classical bse (c-type). |
2017-10-17 |
2023-08-12 |
cattle |
| Grit Priemer, Anne Balkema-Buschmann, Bob Hills, Martin H Groschu. Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE. PloS one. vol 8. issue 6. 2017-10-17. PMID:23805320. |
most importantly, the obex region in the brainstem is not the region with the highest prp(sc) concentrations, but prp(sc) is spread more evenly throughout the entire brain. |
2017-10-17 |
2023-08-12 |
cattle |
| Grit Priemer, Anne Balkema-Buschmann, Bob Hills, Martin H Groschu. Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE. PloS one. vol 8. issue 6. 2017-10-17. PMID:23805320. |
based on these findings, we performed a more detailed western blot study of the anatomical prp(sc) distribution pattern and the biochemical characteristics (molecular mass, glycoprofile as well as pk sensitivity) in ten different anatomical locations of the brain from cattle experimentally challenged with h- or l-type bse, as compared to cattle challenged with c-type bse. |
2017-10-17 |
2023-08-12 |
cattle |
| Grit Priemer, Anne Balkema-Buschmann, Bob Hills, Martin H Groschu. Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE. PloS one. vol 8. issue 6. 2017-10-17. PMID:23805320. |
results of this study revealed distinct differences in the prp(sc) deposition patterns between all three bse forms, while the biochemical characteristics remained stable for each bse type among all analysed brain areas. |
2017-10-17 |
2023-08-12 |
cattle |
| Kathryn J Hilton, Colm Cunningham, Richard A Reynolds, V Hugh Perr. Early Hippocampal Synaptic Loss Precedes Neuronal Loss and Associates with Early Behavioural Deficits in Three Distinct Strains of Prion Disease. PloS one. vol 8. issue 6. 2017-10-10. PMID:23840812. |
astrocyte and microglial activation and protease resistant prion protein (prp(sc)) deposition were assessed in multiple brain regions and showed some strain specificity but also strongly overlapping patterns. |
2017-10-10 |
2023-08-12 |
mouse |
| Kelcey D Dinkel, James B Stanton, David W Boykin, Chad E Stephens, Sally A Madsen-Bouterse, David A Schneide. Antiprion Activity of DB772 and Related Monothiophene- and Furan-Based Analogs in a Persistently Infected Ovine Microglia Culture System. Antimicrobial agents and chemotherapy. vol 60. issue 9. 2017-09-13. PMID:27381401. |
the transmissible spongiform encephalopathies are fatal neurodegenerative disorders characterized by the misfolding of the native cellular prion protein (prp(c)) into the accumulating, disease-associated isoform (prp(sc)). |
2017-09-13 |
2023-08-13 |
Not clear |
| Kelcey D Dinkel, James B Stanton, David W Boykin, Chad E Stephens, Sally A Madsen-Bouterse, David A Schneide. Antiprion Activity of DB772 and Related Monothiophene- and Furan-Based Analogs in a Persistently Infected Ovine Microglia Culture System. Antimicrobial agents and chemotherapy. vol 60. issue 9. 2017-09-13. PMID:27381401. |
previously, we demonstrated the inhibitory activity of db772, a monocationic phenyl-furan-benzimidazole, against prp(sc) accumulation in sheep microglial cells. |
2017-09-13 |
2023-08-13 |
Not clear |
| Kelcey D Dinkel, James B Stanton, David W Boykin, Chad E Stephens, Sally A Madsen-Bouterse, David A Schneide. Antiprion Activity of DB772 and Related Monothiophene- and Furan-Based Analogs in a Persistently Infected Ovine Microglia Culture System. Antimicrobial agents and chemotherapy. vol 60. issue 9. 2017-09-13. PMID:27381401. |
to investigate potential mechanisms of inhibition, effects on prp(c) and prp(sc) were examined. |
2017-09-13 |
2023-08-13 |
Not clear |
| Kelcey D Dinkel, James B Stanton, David W Boykin, Chad E Stephens, Sally A Madsen-Bouterse, David A Schneide. Antiprion Activity of DB772 and Related Monothiophene- and Furan-Based Analogs in a Persistently Infected Ovine Microglia Culture System. Antimicrobial agents and chemotherapy. vol 60. issue 9. 2017-09-13. PMID:27381401. |
while inhibition of total prp(c) was not observed, the results suggest that a potential target for inhibition at biologically relevant concentrations is through prp(c) misfolding to prp(sc) further, sar analysis suggests that two structural elements were associated with micromolar antiprion activity. |
2017-09-13 |
2023-08-13 |
Not clear |
| P Chandrasekaran, R Rajasekara. Detailed computational analysis revealed mutation V210I on PrP induced conformational conversion on β2-α2 loop and α2-α3. Molecular bioSystems. vol 12. issue 10. 2017-07-24. PMID:27523988. |
the development of fatal transmissible spongiform encephalopathies (tse) is associated with the conformational conversion of the normal cellular prion protein, prp(c), into its pathogenic isoform, prp(sc). |
2017-07-24 |
2023-08-13 |
Not clear |
| P Chandrasekaran, R Rajasekara. Detailed computational analysis revealed mutation V210I on PrP induced conformational conversion on β2-α2 loop and α2-α3. Molecular bioSystems. vol 12. issue 10. 2017-07-24. PMID:27523988. |
the present study revealed the structural consequences that induce the conversion of prp(c)→ prp(sc) upon mutation v210i linked with genetic creutzfeldt-jakob disease (cjd) using the classical molecular dynamics (md) approach. |
2017-07-24 |
2023-08-13 |
Not clear |
| P Chandrasekaran, R Rajasekara. Detailed computational analysis revealed mutation V210I on PrP induced conformational conversion on β2-α2 loop and α2-α3. Molecular bioSystems. vol 12. issue 10. 2017-07-24. PMID:27523988. |
in addition, the β2-α2 loop was greatly altered due to the loss of π-π interactions of the residue tyr(169) with phe(175), tye(163), tyr(162), and tyr(218), facilitating more conformational flexibility, which may be involved in the conversion of prp(c)→ prp(sc). |
2017-07-24 |
2023-08-13 |
Not clear |
| P Chandrasekaran, R Rajasekara. Detailed computational analysis revealed mutation V210I on PrP induced conformational conversion on β2-α2 loop and α2-α3. Molecular bioSystems. vol 12. issue 10. 2017-07-24. PMID:27523988. |
this study afforded a detailed structure and dynamic properties of the mutant, which were consistent with the experimental results, providing an insight into the molecular basis for the conversion of prp(c)→ prp(sc), which could be used for the development of antiprion drugs. |
2017-07-24 |
2023-08-13 |
Not clear |
| Ambadi Thody Sabareesan, Jogender Singh, Samrat Roy, Jayant B Udgaonkar, M K Mathe. The Pathogenic A116V Mutation Enhances Ion-Selective Channel Formation by Prion Protein in Membranes. Biophysical journal. vol 110. issue 8. 2017-06-14. PMID:27119637. |
misfolded and aggregated forms of the prion protein (prp(sc)) have been associated with many prion diseases. |
2017-06-14 |
2023-08-13 |
mouse |
| Ambadi Thody Sabareesan, Jogender Singh, Samrat Roy, Jayant B Udgaonkar, M K Mathe. The Pathogenic A116V Mutation Enhances Ion-Selective Channel Formation by Prion Protein in Membranes. Biophysical journal. vol 110. issue 8. 2017-06-14. PMID:27119637. |
a transmembrane form of prp favored by the pathogenic mutation a116v is associated with gerstmann-sträussler-scheinker syndrome, but no accumulation of prp(sc) is detected. |
2017-06-14 |
2023-08-13 |
mouse |
| Atsuko Takeuchi, Atsushi Kobayashi, Piero Parchi, Masahito Yamada, Masanori Morita, Shusei Uno, Tetsuyuki Kitamot. Distinctive properties of plaque-type dura mater graft-associated Creutzfeldt-Jakob disease in cell-protein misfolding cyclic amplification. Laboratory investigation; a journal of technical methods and pathology. vol 96. issue 5. 2017-06-12. PMID:26878132. |
p-dcjd shows distinctive phenotypic features, namely numerous kuru plaques and an abnormal isoform of prion protein (prp(sc)) intermediate in size between types 1 and 2. |
2017-06-12 |
2023-08-13 |
human |