| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Samuel E Saunders, Jason C Bartz, Ronald A Shikiy. Protein misfolding cyclic amplification of prions. Journal of visualized experiments : JoVE. issue 69. 2013-02-15. PMID:23168797. |
pmca is an in vitro technique that takes advantage of this ability using sonication and incubation cycles to produce large amounts of prp(sc), at an accelerated rate, from a system containing excess amounts of prp(c) and minute amounts of the prp(sc) seed(19). |
2013-02-15 |
2023-08-12 |
Not clear |
| Samuel E Saunders, Jason C Bartz, Ronald A Shikiy. Protein misfolding cyclic amplification of prions. Journal of visualized experiments : JoVE. issue 69. 2013-02-15. PMID:23168797. |
this technique has proven to effectively recapitulate the species and strain specificity of prp(sc) conversion from prp(c), to emulate prion strain interference, and to amplify very low levels of prp(sc) from infected tissues, fluids, and environmental samples(6,7,16,23) . |
2013-02-15 |
2023-08-12 |
Not clear |
| Ferdinando Fiorino, Martin Eiden, Armin Giese, Beatrice Severino, Antonella Esposito, Martin H Groschup, Elisa Perissutti, Elisa Magli, Giuseppina Maria Incisivo, Antonio Ciano, Francesco Frecentese, Hans A Kretzschmar, Jens Wagner, Vincenzo Santagada, Giuseppe Caliend. Synthesis of benzamide derivatives and their evaluation as antiprion agents. Bioorganic & medicinal chemistry. vol 20. issue 16. 2013-02-12. PMID:22795751. |
their binding affinity for human prp(c) and inhibition of its conversion into prp(sc) were determined in vitro; moreover, the antiprion activity was assayed by inhibition of prp(sc) accumulation in scrapie-infected mouse neuroblastoma cells (scn2a) and scrapie mouse brain (smb) cells. |
2013-02-12 |
2023-08-12 |
mouse |
| Samuel E Saunders, Shannon L Bartelt-Hunt, Jason C Bart. Resistance of soil-bound prions to rumen digestion. PloS one. vol 7. issue 8. 2013-02-12. PMID:22937149. |
binding to clay appeared to protect noninfectious hamster prp(c) from complete digestion, while both unbound and soil-bound infectious prp(sc) proved highly resistant to rumen digestion. |
2013-02-12 |
2023-08-12 |
Not clear |
| Shanwei Xu, Tim Reuter, Brandon H Gilroyed, Sandor Dudas, Catherine Graham, Norman F Neumann, Aru Balachandran, Stefanie Czub, Miodrag Belosevic, Jerry J Leonard, Tim A McAlliste. Biodegradation of specified risk material and fate of scrapie prions in compost. Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering. vol 48. issue 1. 2013-02-12. PMID:23030385. |
this study investigated the degradation of srm and the fate of scrapie prions (prp(sc)) over 28 days in laboratory-scale composters, with and without feathers in the compost matrices. |
2013-02-12 |
2023-08-12 |
Not clear |
| Shanwei Xu, Tim Reuter, Brandon H Gilroyed, Sandor Dudas, Catherine Graham, Norman F Neumann, Aru Balachandran, Stefanie Czub, Miodrag Belosevic, Jerry J Leonard, Tim A McAlliste. Biodegradation of specified risk material and fate of scrapie prions in compost. Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering. vol 48. issue 1. 2013-02-12. PMID:23030385. |
prior to composting, prp(sc) was detectable in manure with 1-2 log(10) sensitivity, but was not observable after 14 or 28 days of composting. |
2013-02-12 |
2023-08-12 |
Not clear |
| Shanwei Xu, Tim Reuter, Brandon H Gilroyed, Sandor Dudas, Catherine Graham, Norman F Neumann, Aru Balachandran, Stefanie Czub, Miodrag Belosevic, Jerry J Leonard, Tim A McAlliste. Biodegradation of specified risk material and fate of scrapie prions in compost. Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering. vol 48. issue 1. 2013-02-12. PMID:23030385. |
this may have been due to either biological degradation of prp(sc) or the formation of complexes with compost components that precluded its detection. |
2013-02-12 |
2023-08-12 |
Not clear |
| Hermann C Altmeppen, Berta Puig, Frank Dohler, Dana K Thurm, Clemens Falker, Susanne Krasemann, Markus Glatze. Proteolytic processing of the prion protein in health and disease. American journal of neurodegenerative disease. vol 1. issue 1. 2013-02-05. PMID:23383379. |
a prominent, non-physiological property of prpc is the conversion into its pathogenic isoform (prp(sc)) during fatal, transmissible, and neurodegenerative prion diseases. |
2013-02-05 |
2023-08-12 |
human |
| Cleber Eduardo Galvão, Grácia Maria Soares Rosinha, Cristiane Camargo Sanches, Carina Elisei, Flábio Ribeiro Araújo, Gelson Luís Dias Feijó, Roberto Augusto de Almeida Torres, Cleber Oliveira Soare. Polymorphisms of intron 1 and the promoter region at the PRNP gene in BSE-free Caracu cattle. Biochemical genetics. vol 50. issue 9-10. 2013-01-31. PMID:22580793. |
the infectious prion protein prp(sc) is encoded by the prnp gene. |
2013-01-31 |
2023-08-12 |
cattle |
| Cleber Eduardo Galvão, Grácia Maria Soares Rosinha, Cristiane Camargo Sanches, Carina Elisei, Flábio Ribeiro Araújo, Gelson Luís Dias Feijó, Roberto Augusto de Almeida Torres, Cleber Oliveira Soare. Polymorphisms of intron 1 and the promoter region at the PRNP gene in BSE-free Caracu cattle. Biochemical genetics. vol 50. issue 9-10. 2013-01-31. PMID:22580793. |
sequence variants in this gene may affect the formation of prp(sc). |
2013-01-31 |
2023-08-12 |
cattle |
| Shayne A Bellingham, Bradley M Coleman, Andrew F Hil. Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells. Nucleic acids research. vol 40. issue 21. 2013-01-30. PMID:22965126. |
the cellular prion protein, prp(c), and the abnormal infectious form, prp(sc), are found associated with exosomes, which are small 50-130 nm vesicles released from cells. |
2013-01-30 |
2023-08-12 |
Not clear |
| Yoshitaka Yamaguchi, Hironori Miyata, Keiji Uchiyama, Akira Ootsuyama, Sachiko Inubushi, Tsuyoshi Mori, Naomi Muramatsu, Shigeru Katamine, Suehiro Sakaguch. Biological and biochemical characterization of mice expressing prion protein devoid of the octapeptide repeat region after infection with prions. PloS one. vol 7. issue 8. 2013-01-29. PMID:22927985. |
protease-resistant prpΔor, or prp(sc)Δor, was easily detectable but lower in the brains of these mice, compared to that in control wild-type mice. |
2013-01-29 |
2023-08-12 |
mouse |
| Yoshitaka Yamaguchi, Hironori Miyata, Keiji Uchiyama, Akira Ootsuyama, Sachiko Inubushi, Tsuyoshi Mori, Naomi Muramatsu, Shigeru Katamine, Suehiro Sakaguch. Biological and biochemical characterization of mice expressing prion protein devoid of the octapeptide repeat region after infection with prions. PloS one. vol 7. issue 8. 2013-01-29. PMID:22927985. |
however, in their spinal cords, prp(sc)Δor and prion titers were abundant and astrogliosis was as strong as in control wild-type mice. |
2013-01-29 |
2023-08-12 |
mouse |
| Yoshitaka Yamaguchi, Hironori Miyata, Keiji Uchiyama, Akira Ootsuyama, Sachiko Inubushi, Tsuyoshi Mori, Naomi Muramatsu, Shigeru Katamine, Suehiro Sakaguch. Biological and biochemical characterization of mice expressing prion protein devoid of the octapeptide repeat region after infection with prions. PloS one. vol 7. issue 8. 2013-01-29. PMID:22927985. |
we also found that the prp(sc)Δor, including the pre-or residues 23-50, was unusually protease-resistant, indicating that deletion of the or region could cause structural changes to the pre-or region upon prion infection, leading to formation of a protease-resistant structure for the pre-or region. |
2013-01-29 |
2023-08-12 |
mouse |
| Martin Franz, Martin Eiden, Anne Balkema-Buschmann, Justin Greenlee, Hermann Schatzl, Christine Fast, Jürgen Richt, Jan-Peter Hildebrandt, Martin H Groschu. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. The Journal of general virology. vol 93. issue Pt 12. 2013-01-25. PMID:22915695. |
detection of prp(sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. |
2013-01-25 |
2023-08-12 |
mouse |
| Martin Franz, Martin Eiden, Anne Balkema-Buschmann, Justin Greenlee, Hermann Schatzl, Christine Fast, Jürgen Richt, Jan-Peter Hildebrandt, Martin H Groschu. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. The Journal of general virology. vol 93. issue Pt 12. 2013-01-25. PMID:22915695. |
following infection, the protease-resistant, disease-associated isoform of prion protein (prp(sc)) accumulates in the central nervous system and in other tissues. |
2013-01-25 |
2023-08-12 |
mouse |
| Martin Franz, Martin Eiden, Anne Balkema-Buschmann, Justin Greenlee, Hermann Schatzl, Christine Fast, Jürgen Richt, Jan-Peter Hildebrandt, Martin H Groschu. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. The Journal of general virology. vol 93. issue Pt 12. 2013-01-25. PMID:22915695. |
ultrasensitive techniques such as protein misfolding cyclic amplification (pmca) have been developed to detect prp(sc) when present in minuscule amounts that are not readily detected by other diagnostic methods such as immunohistochemistry or western blotting. |
2013-01-25 |
2023-08-12 |
mouse |
| Martin Franz, Martin Eiden, Anne Balkema-Buschmann, Justin Greenlee, Hermann Schatzl, Christine Fast, Jürgen Richt, Jan-Peter Hildebrandt, Martin H Groschu. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. The Journal of general virology. vol 93. issue Pt 12. 2013-01-25. PMID:22915695. |
this study was conducted to determine when and where prp(sc) can be found by pmca in cattle orally challenged with bse. |
2013-01-25 |
2023-08-12 |
mouse |
| Martin Franz, Martin Eiden, Anne Balkema-Buschmann, Justin Greenlee, Hermann Schatzl, Christine Fast, Jürgen Richt, Jan-Peter Hildebrandt, Martin H Groschu. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. The Journal of general virology. vol 93. issue Pt 12. 2013-01-25. PMID:22915695. |
using this protocol, prp(sc) was found in the brain, spinal cord, nerve ganglia, optic nerve and peyer's patches. |
2013-01-25 |
2023-08-12 |
mouse |
| Martin Franz, Martin Eiden, Anne Balkema-Buschmann, Justin Greenlee, Hermann Schatzl, Christine Fast, Jürgen Richt, Jan-Peter Hildebrandt, Martin H Groschu. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. The Journal of general virology. vol 93. issue Pt 12. 2013-01-25. PMID:22915695. |
the presence of prp(sc) was confirmed in adrenal glands, as well as in mesenteric lymph nodes - a finding that was reported recently by another group. |
2013-01-25 |
2023-08-12 |
mouse |