| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| T Ohkuri, T Ueda, M Tsurumaru, T Imot. Evidence for an initiation site for hen lysozyme folding from the reduced form using its dissected peptide fragments. Protein engineering. vol 14. issue 11. 2002-04-26. PMID:11742101. |
however, the mutant fragment i in which cys64-cys80 and cys76-cys94 are lacking owing to the mutation of cys to ala, or the mutant fragment in which trp62 is mutated to gly, did not form the native-like species with fragment ii, because the mutant fragment i derived from mutant lysozymes had no local conformation due to mutations. |
2002-04-26 |
2023-08-12 |
Not clear |
| Kelli Hammond, Gregory A Caputo, Erwin Londo. Interaction of the membrane-inserted diphtheria toxin T domain with peptides and its possible implications for chaperone-like T domain behavior. Biochemistry. vol 41. issue 9. 2002-04-09. PMID:11863463. |
it did not occur at all in the presence a hydrophilic peptide in which alternating ser and gly replaced ala or in the presence of unfolded hydrophilic peptides derived from the a chain of the toxin. |
2002-04-09 |
2023-08-12 |
Not clear |
| T Völkel, T Korn, M Bach, R Müller, R E Konterman. Optimized linker sequences for the expression of monomeric and dimeric bispecific single-chain diabodies. Protein engineering. vol 14. issue 10. 2002-03-18. PMID:11739902. |
the three linkers were randomized in length and sequence using degenerated triplets coding for only six hydrophilic or aliphatic amino acids (thr, ser, asp, asn, gly, ala). |
2002-03-18 |
2023-08-12 |
Not clear |
| BarnesCatherineA Srebalus, Amy E Hilderbrand, Stephen J Valentine, David E Clemme. Resolving isomeric peptide mixtures: a combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library. Analytical chemistry. vol 74. issue 1. 2002-03-13. PMID:11795805. |
a reversed-phase high-performance liquid chromatography (hplc) separation approach has been combined with ion mobility/time-of-flight (tof) mass spectrometry in order to characterize a combinatorial peptide library designed to contain 4000 peptides of the general form nh2-xxx-xxx-xxx-co2h, nh2-ala-xxx-xxx-xxx-co2h, nh2-ser-ala-xxx-xxx-xxx-co2h and nh2-leu-ser-ala-xxx-xxx-xxx-co2h (where xxx represents a randomization over 10 different amino acids: ala, arg, asp, glu, gly, leu, lys, phe, ser, and val). |
2002-03-13 |
2023-08-12 |
Not clear |
| Yan Shen, Aivar Lookene, Solveig Nilsson, Gunilla Olivecron. Functional analyses of human apolipoprotein CII by site-directed mutagenesis: identification of residues important for activation of lipoprotein lipase. The Journal of biological chemistry. vol 277. issue 6. 2002-03-05. PMID:11719505. |
substitution of ala(59) by glycine, or thr(62) and gly(65) by alanine did not change the activation, indicating that these residues are outside the lpl-binding site. |
2002-03-05 |
2023-08-12 |
human |
| R Oliva, L Falcigno, G D'Auria, G Zanotti, L Paolill. Conformation and calcium-binding properties of a bicyclic nonapeptide. Biopolymers. vol 56. issue 1. 2002-02-26. PMID:11582575. |
four carbonyls from the main ring (ala(2), gly(4), ala(6) and gly(8)) point toward it, offering, together with the two carbonyls of the peptide bridge (gly(9) and gammaglu(1)), putative coordinations to the cation. |
2002-02-26 |
2023-08-12 |
Not clear |
| D A Cochran, A J Doi. Effect of the N2 residue on the stability of the alpha-helix for all 20 amino acids. Protein science : a publication of the Protein Society. vol 10. issue 7. 2002-02-21. PMID:11420432. |
the rank order of deltadeltag((relative to ala)) is glu(-), asp(-) > ala > glu(0), leu, val, gln, thr, ile, ser, met, asp(0), his(0), arg, cys, lys, phe > asn, > gly, his(+), pro, tyr. |
2002-02-21 |
2023-08-12 |
Not clear |
| L V Gubareva, R G Webster, F G Hayde. Comparison of the activities of zanamivir, oseltamivir, and RWJ-270201 against clinical isolates of influenza virus and neuraminidase inhibitor-resistant variants. Antimicrobial agents and chemotherapy. vol 45. issue 12. 2002-02-04. PMID:11709315. |
a zanamivir-resistant variant bearing a glu119-to-gly (glu119-->gly) or glu119-->ala substitution in an na (n2) remained susceptible to rwj-270201 and oseltamivir carboxylate. |
2002-02-04 |
2023-08-12 |
Not clear |
| S A Omar, I S Adagu, D W Gump, N P Ndaru, D C Warhurs. Plasmodium falciparum in Kenya: high prevalence of drug-resistance-associated polymorphisms in hospital admissions with severe malaria in an epidemic area. Annals of tropical medicine and parasitology. vol 95. issue 7. 2002-01-24. PMID:11784419. |
these 18 samples also had the mutations associated with resistance to pyrimethamine and sulfadoxine: (asn)51(ile), (cys)59(arg) and (ser)108(asn) of gene dhfr (chromosome 4) and (ala)437(gly) and (lys)540(glu) of dhps (chromosome 8), respectively. |
2002-01-24 |
2023-08-12 |
Not clear |
| K Ohkawa, K Fujii, A Nishida, T Yamauchi, H Ishibashi, H Yamamot. Lysyl oxidase-catalyzed cross-linking and insolubilization reactions of Lys-containing polypeptides and synthetic adhesive proteins. Biomacromolecules. vol 2. issue 3. 2002-01-22. PMID:11710031. |
the km and vmax values and the insolubilization rates varied depending on the lys contents in the substrate polypeptides, which were enriched in gly and ala residues. |
2002-01-22 |
2023-08-12 |
Not clear |
| Gennady M Verkhivker, Djamal Bouzida, Daniel K Gehlhaar, Paul A Rejto, Lana Schaffer, Sandra Arthurs, Anthony B Colson, Stephan T Freer, Veda Larson, Brock A Luty, Tami Marrone, Peter W Ros. Hierarchy of simulation models in predicting structure and energetics of the Src SH2 domain binding to tyrosyl phosphopeptides. Journal of medicinal chemistry. vol 45. issue 1. 2002-01-22. PMID:11754580. |
the results of this study show that small differences in binding free energies between the ala and gly mutants of the pyeei peptide are considerably more difficult to predict than the structure of the bound peptides, indicating that accurate computational prediction of binding affinities still remains a major methodological and technical challenge. |
2002-01-22 |
2023-08-12 |
Not clear |
| S M Pitson, P A Moretti, J R Zebol, M A Vadas, R J D'Andrea, B W Wattenber. A point mutant of human sphingosine kinase 1 with increased catalytic activity. FEBS letters. vol 509. issue 2. 2002-01-17. PMID:11741582. |
surprisingly, a gly(113)-->ala substitution generated a mutant that had 1.7-fold greater catalytic activity than wild-type hsk (hsk(wt)). |
2002-01-17 |
2023-08-12 |
human |
| S M Pitson, P A Moretti, J R Zebol, M A Vadas, R J D'Andrea, B W Wattenber. A point mutant of human sphingosine kinase 1 with increased catalytic activity. FEBS letters. vol 509. issue 2. 2002-01-17. PMID:11741582. |
our data suggests that the gly(113)-->ala mutation increases catalytic efficiency of hsk, probably by inducing a conformational change that increases the efficiency of phosphoryl transfer. |
2002-01-17 |
2023-08-12 |
human |
| S Lee, Y S Cho, C Shim, J Kim, J Choi, S Oh, J Kim, W Zhang, J Le. Aberrant expression of Smad4 results in resistance against the growth-inhibitory effect of transforming growth factor-beta in the SiHa human cervical carcinoma cell line. International journal of cancer. vol 94. issue 4. 2002-01-07. PMID:11745435. |
based on our results, siha cells show low mrna expression of mutated smad4 (gly(230)ala, ala(488)val) and of smads 2, 3, 5 and 6. |
2002-01-07 |
2023-08-12 |
human |
| P Kiatpapan, Y Murook. Construction of an expression vector for propionibacteria and its use in production of 5-aminolevulinic acid by Propionibacterium freudenreichii. Applied microbiology and biotechnology. vol 56. issue 1-2. 2001-12-20. PMID:11499922. |
the recombinant p. freudenreichii accumulated 18.8 mmol/g cells ala in the presence of 1 mm levulinic acid and 30 mm glycine. |
2001-12-20 |
2023-08-12 |
Not clear |
| M Treger, E Westho. Statistical analysis of atomic contacts at RNA-protein interfaces. Journal of molecular recognition : JMR. vol 14. issue 4. 2001-12-19. PMID:11500966. |
the main conclusions are the following: (i) in all three groups of complexes, the most preferred amino acids (arg, asn, ser, lys) and the less preferred ones (ala, ile, leu, val) are the same; trp and cys are rarely observed (respectively 15 and 5 amino acids in the ensemble of interfaces); (ii) of the total number of amino acids located at the interfaces 22% are hydrophobic, 40% charged (positive 32%, negative 8%), 30% polar and 8% are gly; (iii) in ribosomal complexes, phosphate is preferred over ribose, which is preferred over the bases, but there is no significant preference in the other two groups; (iv) there is no significant prevalence of a base type at protein-rna interfaces, but specifically arg and lys display a preference for phosphate over ribose and bases; pro and asn prefer bases over ribose and phosphate; met, phe and tyr prefer ribose over phosphate and bases. |
2001-12-19 |
2023-08-12 |
Not clear |
| V N Kalinin, F A Amosenko, M A Shabanov, L N Lubchenko, S B Hosch, R F Garkavtseva, J R Izbick. Three novel mutations in the RET proto-oncogene. Journal of molecular medicine (Berlin, Germany). vol 79. issue 10. 2001-12-19. PMID:11692159. |
in another tumor two mutations at codons 639 gca(ala)-->gga(gly) and 641 gct(ala)-->cgt(arg) in the exon 11 were observed. |
2001-12-19 |
2023-08-12 |
Not clear |
| Q S Li, J Ogawa, R D Schmid, S Shimiz. Residue size at position 87 of cytochrome P450 BM-3 determines its stereoselectivity in propylbenzene and 3-chlorostyrene oxidation. FEBS letters. vol 508. issue 2. 2001-12-18. PMID:11718725. |
replacement of phe87 with val, ala and gly resulted in decreases in optical purity of produced (r)-(+)-1-phenyl-1-propanol from 90.0 to 37.4, 26.0 and -15.6% e.e., respectively, and in increases in those of produced (r)-(+)-3-chlorostyrene oxide from -61.0 to -38.0, 67.0 and 94.6% e.e., respectively. |
2001-12-18 |
2023-08-12 |
Not clear |
| K Takano, Y Yamagata, K Yutan. Role of amino acid residues in left-handed helical conformation for the conformational stability of a protein. Proteins. vol 45. issue 3. 2001-12-17. PMID:11599030. |
to further examine the role of left-handed residues in the conformational stability of a protein, we constructed ten gly to ala mutant human lysozymes. |
2001-12-17 |
2023-08-12 |
human |
| K Takano, Y Yamagata, K Yutan. Role of amino acid residues in left-handed helical conformation for the conformational stability of a protein. Proteins. vol 45. issue 3. 2001-12-17. PMID:11599030. |
the difference in denaturation gibbs energy (deltadeltag) for the ten gly to ala mutants ranged from + 1.9 to -7.5 kj/mol, indicating that the effect of the mutation depends on the environment of the residue. |
2001-12-17 |
2023-08-12 |
human |