| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Richard Rubenstein, Allen Chiu, Binggong Chang, Thomas Wisniewsk. Role of CD40 in prion disease and the immune response to recombinant PrP. Journal of neuroimmunology. vol 257. issue 1-2. 2013-05-21. PMID:23419881. |
our results suggest that cd40: (i) results in slower disease progression and scrapie strain-specific differences in incubation periods, (ii) does not affect the level of scrapie strain-specific prp(sc), (iii) does not influence disease-associated neuropathology, but (iv), as expected, is required to mount an immune response generating anti-prp igg antibodies. |
2013-05-21 |
2023-08-12 |
mouse |
| Fabien Corbière, Cécile Perrin-Chauvineau, Caroline Lacroux, Pierrette Costes, Myriam Thomas, Isabelle Brémaud, Samuel Martin, Séverine Lugan, Christophe Chartier, François Schelcher, Francis Barillet, Olivier Andreolett. PrP-associated resistance to scrapie in five highly infected goat herds. The Journal of general virology. vol 94. issue Pt 1. 2013-05-15. PMID:23100359. |
on the basis of prp(sc) detection in the central nervous system and in various lymphoid tissues, 301 of 1343 goats were found to be scrapie infected. |
2013-05-15 |
2023-08-12 |
Not clear |
| Ester Vázquez-Fernández, Jana Alonso, Miguel A Pastrana, Adriana Ramos, Lothar Stitz, Enric Vidal, Irina Dynin, Benjamin Petsch, Christopher J Silva, Jesús R Requen. Structural organization of mammalian prions as probed by limited proteolysis. PloS one. vol 7. issue 11. 2013-05-13. PMID:23185550. |
elucidation of the structure of prp(sc) continues to be one major challenge in prion research. |
2013-05-13 |
2023-08-12 |
mouse |
| Ester Vázquez-Fernández, Jana Alonso, Miguel A Pastrana, Adriana Ramos, Lothar Stitz, Enric Vidal, Irina Dynin, Benjamin Petsch, Christopher J Silva, Jesús R Requen. Structural organization of mammalian prions as probed by limited proteolysis. PloS one. vol 7. issue 11. 2013-05-13. PMID:23185550. |
thus, limited proteolysis has been successfully used to pinpoint flexible regions within prion multimers (prp(sc)). |
2013-05-13 |
2023-08-12 |
mouse |
| Ester Vázquez-Fernández, Jana Alonso, Miguel A Pastrana, Adriana Ramos, Lothar Stitz, Enric Vidal, Irina Dynin, Benjamin Petsch, Christopher J Silva, Jesús R Requen. Structural organization of mammalian prions as probed by limited proteolysis. PloS one. vol 7. issue 11. 2013-05-13. PMID:23185550. |
in order to surmount these difficulties we analyzed prp(sc) from transgenic mice expressing prion protein (prp) lacking the gpi membrane anchor. |
2013-05-13 |
2023-08-12 |
mouse |
| Ester Vázquez-Fernández, Jana Alonso, Miguel A Pastrana, Adriana Ramos, Lothar Stitz, Enric Vidal, Irina Dynin, Benjamin Petsch, Christopher J Silva, Jesús R Requen. Structural organization of mammalian prions as probed by limited proteolysis. PloS one. vol 7. issue 11. 2013-05-13. PMID:23185550. |
gpi-less prp(sc) samples were digested with pk. |
2013-05-13 |
2023-08-12 |
mouse |
| Ester Vázquez-Fernández, Jana Alonso, Miguel A Pastrana, Adriana Ramos, Lothar Stitz, Enric Vidal, Irina Dynin, Benjamin Petsch, Christopher J Silva, Jesús R Requen. Structural organization of mammalian prions as probed by limited proteolysis. PloS one. vol 7. issue 11. 2013-05-13. PMID:23185550. |
the first 10 peptides (to position 153), match very well with pk cleavage sites we previously identified in wild type prp(sc). |
2013-05-13 |
2023-08-12 |
mouse |
| Ester Vázquez-Fernández, Jana Alonso, Miguel A Pastrana, Adriana Ramos, Lothar Stitz, Enric Vidal, Irina Dynin, Benjamin Petsch, Christopher J Silva, Jesús R Requen. Structural organization of mammalian prions as probed by limited proteolysis. PloS one. vol 7. issue 11. 2013-05-13. PMID:23185550. |
these results reinforce the hypothesis that the structure of prp(sc) consists of a series of highly pk-resistant β-sheet strands connected by short flexible pk-sensitive loops and turns. |
2013-05-13 |
2023-08-12 |
mouse |
| Ester Vázquez-Fernández, Jana Alonso, Miguel A Pastrana, Adriana Ramos, Lothar Stitz, Enric Vidal, Irina Dynin, Benjamin Petsch, Christopher J Silva, Jesús R Requen. Structural organization of mammalian prions as probed by limited proteolysis. PloS one. vol 7. issue 11. 2013-05-13. PMID:23185550. |
a sizeable c-terminal stretch of prp(sc) is highly resistant to pk and therefore perhaps also contains β-sheet secondary structure. |
2013-05-13 |
2023-08-12 |
mouse |
| Abdelazim E Elhelaly, Yasuo Inoshima, Naotaka Ishigur. Alteration of cell responses to PrP(Sc) in prolonged cell culture and its effect on transmission of PrP(Sc) to neural cells. Archives of virology. vol 158. issue 3. 2013-05-01. PMID:23160705. |
alteration of cell responses to prp(sc) in prolonged cell culture and its effect on transmission of prp(sc) to neural cells. |
2013-05-01 |
2023-08-12 |
Not clear |
| Abdelazim E Elhelaly, Yasuo Inoshima, Naotaka Ishigur. Alteration of cell responses to PrP(Sc) in prolonged cell culture and its effect on transmission of PrP(Sc) to neural cells. Archives of virology. vol 158. issue 3. 2013-05-01. PMID:23160705. |
the mechanisms and processes of the uptake, intracellular trafficking and intercellular spread of prp(sc) and its transfer to neural cells are not clearly defined. |
2013-05-01 |
2023-08-12 |
Not clear |
| Abdelazim E Elhelaly, Yasuo Inoshima, Naotaka Ishigur. Alteration of cell responses to PrP(Sc) in prolonged cell culture and its effect on transmission of PrP(Sc) to neural cells. Archives of virology. vol 158. issue 3. 2013-05-01. PMID:23160705. |
the role of these cell types in the accumulation and transfer of prp(sc) to neural cells was investigated following short and prolonged exposure to the chandler and obihiro strains of scrapie prp(sc) for up to 28 days. |
2013-05-01 |
2023-08-12 |
Not clear |
| Abdelazim E Elhelaly, Yasuo Inoshima, Naotaka Ishigur. Alteration of cell responses to PrP(Sc) in prolonged cell culture and its effect on transmission of PrP(Sc) to neural cells. Archives of virology. vol 158. issue 3. 2013-05-01. PMID:23160705. |
after transient degradation phases, certain immune, intestinal and neural cells accumulated prp(sc) for up to 28 days postinfection. |
2013-05-01 |
2023-08-12 |
Not clear |
| Abdelazim E Elhelaly, Yasuo Inoshima, Naotaka Ishigur. Alteration of cell responses to PrP(Sc) in prolonged cell culture and its effect on transmission of PrP(Sc) to neural cells. Archives of virology. vol 158. issue 3. 2013-05-01. PMID:23160705. |
our results suggest that some of these cell types may have a role in prp(sc) accumulation and intercellular spread of prp(sc) infection to neural cells in vivo. |
2013-05-01 |
2023-08-12 |
Not clear |
| Richard Rubenstei. Proteomic analysis of prion diseases: creating clarity or causing confusion? Electrophoresis. vol 33. issue 24. 2013-04-29. PMID:23161058. |
there are both human and animal forms of the disease and all are associated with the conversion of a normal host-coded cellular prion protein (prp(c) ) into an abnormal protease-resistant isoform (prp(sc) ). |
2013-04-29 |
2023-08-12 |
human |
| Richard Rubenstei. Proteomic analysis of prion diseases: creating clarity or causing confusion? Electrophoresis. vol 33. issue 24. 2013-04-29. PMID:23161058. |
although methodologies are sensitive and specific for postmortem disease diagnosis, the use of prp(sc) as a preclinical or general biomarker for surveillance is difficult, due to the fact that it is present in extremely small amounts in accessible tissues or body fluids such as blood, urine, saliva, and cerebrospinal fluid. |
2013-04-29 |
2023-08-12 |
human |
| Richard Rubenstei. Proteomic analysis of prion diseases: creating clarity or causing confusion? Electrophoresis. vol 33. issue 24. 2013-04-29. PMID:23161058. |
recently, amplification techniques have been developed, which have enabled increased sensitivity for prp(sc) detection. |
2013-04-29 |
2023-08-12 |
human |
| Richard Rubenstei. Proteomic analysis of prion diseases: creating clarity or causing confusion? Electrophoresis. vol 33. issue 24. 2013-04-29. PMID:23161058. |
accordingly, the development of new diagnostic tests that do not rely on prp(sc) and proteinase k digestion is desirable. |
2013-04-29 |
2023-08-12 |
human |
| Richard Rubenstei. Proteomic analysis of prion diseases: creating clarity or causing confusion? Electrophoresis. vol 33. issue 24. 2013-04-29. PMID:23161058. |
the search for biomarkers (other than prp(sc) ) as tools for diagnosis of prion diseases has a long history. |
2013-04-29 |
2023-08-12 |
human |
| Pavlina Sobrova, Marketa Ryvolova, Vojtech Adam, Rene Kize. Capillary electromigration based techniques in diagnostics of prion protein caused diseases. Electrophoresis. vol 33. issue 24. 2013-04-29. PMID:23161211. |
presently, diagnostic methods such as protein misfolding cyclic amplification, conformation-dependent immunoassay, dissociation-enhanced lanthanide fluorescent immunoassay, fluorescence correlation spectroscopy, and/or flow microbead immunoassay are used for abnormal prion protein (prp(sc) ) detection. |
2013-04-29 |
2023-08-12 |
human |