| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jean-Daniel Horisberger, Solange Kharoubi-Hess, Saïda Guennoun, Olivier Michieli. The fourth transmembrane segment of the Na,K-ATPase alpha subunit: a systematic mutagenesis study. The Journal of biological chemistry. vol 279. issue 28. 2004-08-24. PMID:15123699. |
accessibility, determined as a 2-aminoethyl-methanethiosulfonate-induced reduction of the transport activity or as inhibition of the membrane conductance after palytoxin treatment, was observed for the following positions: phe(323), ile(322), gly(326), ala(330), pro(333), glu(334), and gly(335). |
2004-08-24 |
2023-08-12 |
Not clear |
| J Wu, B H. [Purification and properties of beta-D-mannanase from Nocardioform actinomycetes]. Wei sheng wu xue bao = Acta microbiologica Sinica. vol 40. issue 1. 2004-08-10. PMID:12548881. |
the enzyme was optimally active at ph 8 and 75 degrees c and showed stability at ph range of 6.5 to 12 at the temperature below 60 degrees c. the amino acid composition analysis of the enzyme proved that there were large amount of gly, asp, ala and glu. |
2004-08-10 |
2023-08-12 |
Not clear |
| El Houssine Boufous, Christian Vadeboncoeu. Purification and characterization of the Streptococcus salivarius methionine aminopeptidase (MetAP). Biochimie. vol 85. issue 10. 2004-08-10. PMID:14644554. |
the native enzyme eluted from a superdex column as a protein with a molecular mass of 30.6 kda and cleaved n-terminal met of peptide only when the penultimate amino acid was gly, ala, ser, val, pro, or thr. |
2004-08-10 |
2023-08-12 |
Not clear |
| Xing Han, Wenjun Kan. Sequence analysis and membrane partitioning energies of alpha-helical antimicrobial peptides. Bioinformatics (Oxford, England). vol 20. issue 6. 2004-08-04. PMID:14764568. |
the results showed that aliphatic amino acids gly, leu, ala, ile and two positively charged amino acids lys and arg were composed of more than 63% of the first 20 residues of alphaamps. |
2004-08-04 |
2023-08-12 |
Not clear |
| Mark Miskolzie, Scott Lucyk, George Kotovyc. NMR conformational studies of micelle-bound orexin-B: a neuropeptide involved in the sleep/awake cycle and feeding regulation. Journal of biomolecular structure & dynamics. vol 21. issue 3. 2004-08-02. PMID:14616030. |
have shown that ala substitution from gly(24) to met(28) or d-amino acid substitution from ala(23) to met(28) causes a significant reduction in the potency of orexin-b for both ox(1)r and ox(2)r receptors. |
2004-08-02 |
2023-08-12 |
human |
| Sigrid Gåseidnes, Bjørnar Synstad, Xiaohong Jia, Hege Kjellesvik, Gert Vriend, Vincent G H Eijsin. Stabilization of a chitinase from Serratia marcescens by Gly-->Ala and Xxx-->Pro mutations. Protein engineering. vol 16. issue 11. 2004-07-23. PMID:14631073. |
stabilization of a chitinase from serratia marcescens by gly-->ala and xxx-->pro mutations. |
2004-07-23 |
2023-08-12 |
Not clear |
| Sigrid Gåseidnes, Bjørnar Synstad, Xiaohong Jia, Hege Kjellesvik, Gert Vriend, Vincent G H Eijsin. Stabilization of a chitinase from Serratia marcescens by Gly-->Ala and Xxx-->Pro mutations. Protein engineering. vol 16. issue 11. 2004-07-23. PMID:14631073. |
this paper describes attempts to increase the kinetic stability of chitinase b from serratia marcescens (chib) by the introduction of semi-automatically designed rigidifying mutations of the gly-->ala and xxx-->pro type. |
2004-07-23 |
2023-08-12 |
Not clear |
| E Tarelli, P H Corra. Ammonia cleaves polypeptides at asparagine proline bonds. The journal of peptide research : official journal of the American Peptide Society. vol 62. issue 6. 2004-07-23. PMID:14632927. |
other asn-x bonds where x = tyr, gln, ile, glu, ala, gly, asn or phe did not exhibit any peptide bond cleavage, whereas when x = leu, thr and ser partial cleavage was observed. |
2004-07-23 |
2023-08-12 |
Not clear |
| Stanley C Kwok, Robert S Hodge. Stabilizing and destabilizing clusters in the hydrophobic core of long two-stranded alpha-helical coiled-coils. The Journal of biological chemistry. vol 279. issue 20. 2004-07-21. PMID:15020585. |
three types of regions were present in the hydrophobic core of both proteins: stabilizing clusters and destabilizing clusters, defined as three or more consecutive core residues of either stabilizing (leu, ile, val, met, phe, and tyr) or destabilizing (gly, ala, cys, ser, thr, asn, gln, asp, glu, his, arg, lys, and trp) residues, and intervening regions that consist of both stabilizing and destabilizing residues in the hydrophobic core but no clusters. |
2004-07-21 |
2023-08-12 |
Not clear |
| Ana Caballero-Herrera, Lennart Nilsso. Molecular dynamics simulations of the E1/E2 transmembrane domain of the Semliki Forest virus. Biophysical journal. vol 85. issue 6. 2004-07-20. PMID:14645057. |
these were small and medium residues as gly, ala, ser, and leu, which also had the possibility to form interhelical calpha-h...o hydrogen bonds. |
2004-07-20 |
2023-08-12 |
Not clear |
| Tobias G Köllner, Christiane Schnee, Jonathan Gershenzon, Jörg Degenhard. The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes. The Plant cell. vol 16. issue 5. 2004-07-19. PMID:15075399. |
the differences in the stereoselectivity of tps4 and tps5 are determined by four amino acid substitutions with the most important being a gly instead of an ala residue at position 409 at the catalytic site of the enzyme. |
2004-07-19 |
2023-08-12 |
Not clear |
| Emily R Slepkov, Signy Chow, M Joanne Lemieux, Larry Fliege. Proline residues in transmembrane segment IV are critical for activity, expression and targeting of the Na+/H+ exchanger isoform 1. The Biochemical journal. vol 379. issue Pt 1. 2004-07-16. PMID:14680478. |
pro167 and pro168 were mutated to gly, ala or cys, and pro178 was mutated to ala. pro168 and pro178 mutant proteins were expressed at levels similar to wild-type nhe1 and were targeted to the plasma membrane. |
2004-07-16 |
2023-08-12 |
Not clear |
| Sushmita D Lahiri, Guofeng Zhang, Jianying Dai, Debra Dunaway-Mariano, Karen N Alle. Analysis of the substrate specificity loop of the HAD superfamily cap domain. Biochemistry. vol 43. issue 10. 2004-07-12. PMID:15005616. |
to define the role of the conserved gly in the structure and function of the cap domain loop of the had superfamily members phosphonoacetaldehyde hydrolase and beta-phosphoglucomutase, the gly was mutated to pro, val, or ala. |
2004-07-12 |
2023-08-12 |
Not clear |
| Frederick F Samaha, Ronald C Rubenstein, Wusheng Yan, Mohan Ramkumar, Daniel I Levy, Yoon J Ahn, Shaohu Sheng, Thomas R Kleyma. Functional polymorphism in the carboxyl terminus of the alpha-subunit of the human epithelial sodium channel. The Journal of biological chemistry. vol 279. issue 23. 2004-07-08. PMID:15069064. |
whole cell amiloride-sensitive currents in xenopus oocytes expressing wild type channels (alphat663betagamma) were significantly approximately 1.3-2.0-fold higher than currents measured in oocytes expressing channels with an ala, gly or leu, or lys at position alpha663. |
2004-07-08 |
2023-08-12 |
mouse |
| Rajamani Sudha, Lavanya Anantharaman, Mylavarapu V S Sivaram, Neda Mirsamadi, Devapriya Choudhury, Nirmal K Lohiya, Rasik B Gupta, Rajendra P Ro. Linkage of interactions in sickle hemoglobin fiber assembly: inhibitory effect emanating from mutations in the AB region of the alpha-chain is annulled by a mutation at its EF corner. The Journal of biological chemistry. vol 279. issue 19. 2004-06-15. PMID:14982923. |
we have examined the role of dynamics of ab/gh region on hbs polymerization through simultaneous replacement of non-contact ala(19) and ala(21) of the ab corner with more flexible gly or rigid alpha-aminoisobutyric acid (aib) residues. |
2004-06-15 |
2023-08-12 |
Not clear |
| Rajamani Sudha, Lavanya Anantharaman, Mylavarapu V S Sivaram, Neda Mirsamadi, Devapriya Choudhury, Nirmal K Lohiya, Rasik B Gupta, Rajendra P Ro. Linkage of interactions in sickle hemoglobin fiber assembly: inhibitory effect emanating from mutations in the AB region of the alpha-chain is annulled by a mutation at its EF corner. The Journal of biological chemistry. vol 279. issue 19. 2004-06-15. PMID:14982923. |
molecular dynamics simulation studies of alpha-chains indicated that coordinated motion of ab and gh region residues present in native (ala) as well as in aib mutant was disrupted in the gly mutant. |
2004-06-15 |
2023-08-12 |
Not clear |
| Sanguk Kim, Aaron K Chamberlain, James U Bowi. Membrane channel structure of Helicobacter pylori vacuolating toxin: role of multiple GXXXG motifs in cylindrical channels. Proceedings of the National Academy of Sciences of the United States of America. vol 101. issue 16. 2004-06-15. PMID:15067113. |
in our model, gly residues in gxxxg motifs pack against small ala or val side chains to generate the pore. |
2004-06-15 |
2023-08-12 |
human |
| Tara L Pukala, Craig S Brinkworth, John A Carver, John H Bowi. Investigating the importance of the flexible hinge in caerin 1.1: solution structures and activity of two synthetically modified caerin peptides. Biochemistry. vol 43. issue 4. 2004-05-20. PMID:14744137. |
to evaluate the importance of the two pro residues, they were replaced with either ala or gly. |
2004-05-20 |
2023-08-12 |
Not clear |
| Tara L Pukala, Craig S Brinkworth, John A Carver, John H Bowi. Investigating the importance of the flexible hinge in caerin 1.1: solution structures and activity of two synthetically modified caerin peptides. Biochemistry. vol 43. issue 4. 2004-05-20. PMID:14744137. |
the resulting structures indicate that the central hinge angle decreases significantly upon replacement of the pro residues with gly and to a further extent with ala. |
2004-05-20 |
2023-08-12 |
Not clear |
| Tara L Pukala, Craig S Brinkworth, John A Carver, John H Bowi. Investigating the importance of the flexible hinge in caerin 1.1: solution structures and activity of two synthetically modified caerin peptides. Biochemistry. vol 43. issue 4. 2004-05-20. PMID:14744137. |
in a broader context, the use of pro, gly, and ala variants of caerin 1.1 has enabled the relationship between conformational flexibility and activity to be directly investigated in a systematic manner. |
2004-05-20 |
2023-08-12 |
Not clear |